Has Covid-19 been isolated? YES!!
So in today’s Medium article, I’ll ONLY be discussing the factual proof available that Covid-19 HAS been isolated.
I’m addressing this due to the rise of false claims that Covid-19 hasn’t been isolated, which has been circulating yet again on social media and other parts of the internet. So sit back, enjoy the read and please share this article with people who state the false claim.
➡ The Whole Covid-19 Genome
The best piece of proof available right now that it has been isolated is the complete genome sequence of this RNA virus which is publicly available for anyone to visit and have a read through. (Source: https://www.ncbi.nlm.nih.gov/nuccore/NC_045512)
➡ First case in the USA
There’s even evidence of Covid-19 isolation from a peer-reviewed study on very first documented case of SARS-CoV-2 on USA soil. (Source: https://www.nejm.org/doi/10.1056/NEJMoa2001191)
➡ Full detail on isolation
So from the above patient the CDC on a very good study on Covid-19 isolation and all aspects that were undertaken into isolation and more. (Source: https://wwwnc.cdc.gov/eid/article/26/6/pdfs/20-0516.pdf)
➡ Other resources
There are links to multiple databases of sequences, structures, and more on the NCBI site. (Source: https://www.ncbi.nlm.nih.gov/sars-cov-2/)
Getting all of the above requires isolates. Experiments that pharmaceutical companies and other researchers conduct tests, whether given mutations impact the efficacy of therapeutics. So this link is another very good resource to draw information from. (Source: https://opendata.ncats.nih.gov/covid19/)
➡ Other Links of evidence for isolation
Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells -https://doi.org/10.1073/pnas.2002589117
Sascha Harper Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
Osong Public Health Res Perspect. 2020 Feb; 11(1): 3–7.
doi: 10.24171/j.phrp.2020.11.1.02
PMCID: PMC7045880
PMID: 32149036
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045880/#__ffn_sectitle
Pavel STI, Yetiskin H, Aydin G, Holyavkin C, Uygut MA, Dursun ZB, et al. (2020)
DOI: 10.1371/journal.pone.0238614
PMID: 32936826
PMCID: PMC7494126.
Published: September 16, 2020
“Isolation and characterization of severe acute respiratory syndrome coronavirus 2 in Turkey.”
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238614
Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient, Emerging Microbes & Infections
https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1760144
Viral propagation and quantitation of severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. A) Two virus passage 4 stocks (black and gray circles) were quantified by using plaque assay at day 2 (solid circles) and day 3 (open circles) postinfection of Vero E6 and Vero CCL81 cells. Plaque morphology for virus on Vero E6 and Vero CCL81 at day 2 and day 3 postinoculation. C) Cell monolayers 2 days postinfection of Vero E6 (top) and Vero CCL81 (bottom) at 3 dilutions. — https://wwwnc.cdc.gov/eid/article/26/6/20-0516-f2
Transmission electron microscopy imaging of SARS-CoV-2 — https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224615/
Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high-resolution scanning electron microscopy — https://www.nature.com/articles/s41598-020-73162-5
Full size image: https://www.nature.com/articles/s41598-020-73162-5/figures/1
Electron microscopy of SARS-CoV in inoculum, clinical samples, and tissue samples of experimentally infected cynomolgus macaques — https://www.researchgate.net/figure/Electron-microscopy-of-SARS-CoV-in-inoculum-clinical-samples-and-tissue-samples-of_fig4_295413709
Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient, Emerging Microbes & Infections
https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1760144
Complete Genome Sequence of a SARS-CoV-2 Strain Isolated in Northern Germany
PMCID: PMC7272567
PMID: 32499358
doi: 10.1128/MRA.00520–20
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272567/
Complete Genome Sequence of a 2019 Novel Coronavirus (SARS-CoV-2) Strain Isolated in Nepal
DOI: 10.1128/MRA.00169–20
https://mra.asm.org/content/9/11/e00169-20
Complete Genome Sequence of a 2019 Novel Coronavirus (SARS-CoV-2) Strain Causing a COVID-19 Case in Morocco
doi: 10.1128/MRA.00633–20
PMID: 32616647
PMCID: PMC7330249
https://pubmed.ncbi.nlm.nih.gov/32616647/
Near-Complete Genome Sequence of a 2019 Novel Coronavirus (SARS-CoV-2) Strain Causing a COVID-19 Case in Peru
doi: 10.1128/MRA.00303–20
PMID: 32381617
PMCID: PMC7206495
https://pubmed.ncbi.nlm.nih.gov/32381617/
Complete Genome Sequence of a SARS-CoV-2 Strain Isolated in Northern Germany
PMID: 32499358
PMCID: PMC7272567
DOI: 10.1128/MRA.00520–20
https://pubmed.ncbi.nlm.nih.gov/32499358/
Complete Genome Sequences of Five 2019 Novel Coronavirus (SARS-CoV-2) Strains Isolated in Bangladesh
PMID: 32732236
PMCID: PMC7393965
DOI: 10.1128/MRA.00692–20
https://pubmed.ncbi.nlm.nih.gov/32732236/
Complete Genome Sequence of a Novel Coronavirus (SARS-CoV-2) Isolate from Bangladesh
PMID: 32527780
PMCID: PMC7291105
DOI: 10.1128/MRA.00568–20
https://pubmed.ncbi.nlm.nih.gov/32527780/
Complete Genome Sequence of a SARS-CoV-2 Strain Isolated in Gilgit, Pakistan
PMID: 33795347
DOI: 10.1128/MRA.01151–20
https://pubmed.ncbi.nlm.nih.gov/33795347/
Complete Genome Sequences of Two SARS-CoV-2 Isolates from Egypt
PMID: 32467284
PMCID: PMC7256271
DOI: 10.1128/MRA.00489–20
https://pubmed.ncbi.nlm.nih.gov/32467284/
Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
PMID: 32149036
PMCID: PMC7045880
DOI: 10.24171/j.phrp.2020.11.1.02
https://pubmed.ncbi.nlm.nih.gov/32149036/
Complete Genome Sequences of Two SARS-CoV-2 Isolates from Early Manifestations of COVID-19 in Israel
PMID: 32646911
PMCID: PMC7348029
DOI: 10.1128/MRA.00677–20
https://pubmed.ncbi.nlm.nih.gov/32646911/
Detection and Isolation of SARS-CoV-2 in Serum, Urine, and Stool Specimens of COVID-19 Patients from the Republic of Korea
PMID: 32528816
PMCID: PMC7282421
DOI: 10.24171/j.phrp.2020.11.3.02
https://pubmed.ncbi.nlm.nih.gov/32528816/
New Images of Novel Coronavirus SARS-CoV-2: https://www.niaid.nih.gov/news-events/novel-coronavirus-sarscov2-images
➡ Conclusion
Very short conclusion. COVID-19 HAS BEEN ISOLATED, TOO MUCH EVIDENCE IS AVAILABLE.
💥 Thanks for reading, Lawrence. Please consider a small contribution, in the form of a beer as all articles are created in my small amount of spare time: https://www.buymeacoffee.com/LawrenceRob